Rabbit Fibroblast Growth Factor 7 ELISA Kit | FGF7 elisa kit

Rabbit Fibroblast Growth Factor 7 ELISA Kit

Cat. Num. AAA730726

• Gene Names: FGF7; KGF; HBGF-7
• Reactivity: Rabbit
• Synonyms: Fibroblast Growth Factor 7; Rabbit Fibroblast Growth Factor 7 ELISA Kit; FGF7 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This FGF7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit FGF7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF7 elisa kit

Principle of the assay: FGF7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FGF7 antibody and an FGF7-HRP conjugate. The assay sample and buffer are incubated together with FGF7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FGF7 concentration since FGF7 from samples and FGF7-HRP conjugate compete for the anti-FGF7 antibody binding site. Since the number of sites is limited, as more sites are occupied by FGF7 from the sample, fewer sites are left to bind FGF7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FGF7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FGF7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 49456957
• NCBI GeneID: 2252
• UniProt Accession #: Q6FGV5; Q96FG5; H0YNY5; P21781
• Molecular Weight: 11,520 Da
• NCBI Official Full Name: FGF7, partial
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: fibroblast growth factor 7; FGF-7; keratinocyte growth factor; heparin-binding growth factor 7
• UniProt Protein Name: Fibroblast growth factor 7
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF; FGF-7; HBGF-7
• UniProt Entry Name: FGF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; epidermis development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hair follicle morphogenesis; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation