Fibroblast Growth Factor 10 (FGF10) Recombinant Protein | FGF10 recombinant protein

Recombinant Fibroblast Growth Factor 10 (FGF10)

Cat. Num. AAA2011415

• Gene Names: Fgf10; FGF-10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibroblast Growth Factor 10 (FGF10); Recombinant Fibroblast Growth Factor 10 (FGF10); FGF10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-QALG QDMVSPEATN SSSSSSSSSS SSSFSSPSSA GRHVRSYNHL QGDVRWRKLF SFTKYFLKIE KNGKVSGTKK ENCPYSILEI TSVEIGVVAV KAINSNYYLA MNKKGKLYGS KEFNNDCKLK ERIEENGYNT YASFNWQHNG RQMYVALNGK GAPRRGQKTR RKNTSAHFLP MVVHS
• Sequence Length: 215

• Applicable Applications for FGF10 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln37~Ser215 (Accession # P70492) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978837
• NCBI GeneID: 25443
• NCBI Accession #: NP_037083.1
• NCBI GenBank Nucleotide #: NM_012951.1
• UniProt Accession #: P70492
• Molecular Weight: 47.1kDa
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: Fgf10
• NCBI Official Synonym Symbols: FGF-10
• NCBI Protein Information: fibroblast growth factor 10
• UniProt Protein Name: Fibroblast growth factor 10
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_RAT

NCBI Description

binds the KGFR receptor, a splice variant of FGFR-2; may contribute to tissue regeneration and angiogenesis after acute pancreatitis [RGD, Feb 2006]

Uniprot Description

Function: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.

Subunit structure: Interacts with FGFR1 and FGFR2. Interacts with FGFBP1

By similarity.

Subcellular location: Secreted

Potential.

Tissue specificity: Preferentially expressed in the lung in adults.

Sequence similarities: Belongs to the heparin-binding growth factors family.