acidic Fibroblast Growth Factor Recombinant Protein | aFGF recombinant protein

Human acidic Fibroblast Growth Factor Recombinant

Cat. Num. AAA553019

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: acidic Fibroblast Growth Factor; Human acidic Fibroblast Growth Factor Recombinant; HBGF-1; afgf; Beta-endothelial cell growth factor; aFGF recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant acidic FGF was lyophilized from a 0.2 um filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
• Sequence: MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
• Sequence Length: 155

• Domain: HBGF/IL1
• Host Note: Optimized DNA sequence encoding Human acidic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human aFGF, generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated mass of 16 kDa.; ; Recombinant FGF acidic is a disulfide-linked monomer protein consisting of 141 amino acids and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for aFGF recombinant protein

aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.

Product Categories/Family for aFGF recombinant protein

Cytokines

References

• expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas .J. Clin. Pathol., Jan 2001; 54: 37 - 41.
• requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus .J. Biol. Chem., Apr 2000; 275: 11972.
• angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia .Am J Physiol Heart Circ Physiol, Mar 1998; 274: H930 - H936.
• the fibroblast growth factor receptor-1 is necessary for the induction of neurite outgrowth in pc12 cells by aFGF .J. Neurosci., Aug 1996; 16: 4579.
• the major acidic fibroblast growth factor (aFGF) -stimulated phosphoprotein from bovine liver plasma membranes has aFGF-stimulated kinase, autoadenylylation, and alkaline nucleotide phosphodiesterase activities .J. Biol. Chem., Dec 1993; 268: 27318 - 27326.