FFAR2 / Free Fatty Acid Receptor 2 Recombinant Protein | FFAR2 recombinant protein

Human FFAR2 / Free Fatty Acid Receptor 2 Recombinant Protein

• Gene Names: FFAR2; FFA2R; GPR43
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: FFAR2 / Free Fatty Acid Receptor 2; Human FFAR2 / Free Fatty Acid Receptor 2 Recombinant Protein; Free fatty acid receptor 2; G-protein coupled receptor 43; FFA2; GPCR43; GPR43; FFAR2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 330

• Applicable Applications for FFAR2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with FCN1 (via Fibrinogen C-terminal domain).
• Entry Function: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097). Among SCFAs, the fatty acids containing less than 6 carbons, the most potent activators are probably acetate, propionate and butyrate (PubMed:12496283, PubMed:12711604). Exhibits a SCFA-independent constitutive G protein-coupled receptor activity (PubMed:23066016).
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4885333
• NCBI GeneID: 2867
• NCBI Accession #: NP_005297.1
• NCBI GenBank Nucleotide #: NP_005297.1
• UniProt Accession #: O15552
• NCBI Official Full Name: free fatty acid receptor 2
• NCBI Official Synonym Full Names: free fatty acid receptor 2
• NCBI Official Symbol: FFAR2
• NCBI Official Synonym Symbols: FFA2R; GPR43
• NCBI Protein Information: free fatty acid receptor 2
• UniProt Protein Name: Free fatty acid receptor 2
• Protein Family: Free fatty acid receptor
• UniProt Gene Name: FFAR2
• UniProt Synonym Gene Names: GPR43
• UniProt Entry Name: FFAR2_HUMAN

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for short chain free fatty acids and may be involved in the inflammatory response and in regulating lipid plasma levels. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR2: Receptor for short chain fatty acids through a G(i)- protein-mediated inhibition of adenylyl cyclase and elevation of intracellular calcium. The rank order of potency for agonists of this receptor is acetate= propionate = butyrate > pentanoate = formate. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; lipid binding

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; sequestering of lipid; mucosal immune response; regulation of acute inflammatory response; cell surface pattern recognition receptor signaling pathway; positive regulation of cytokine production during immune response; positive regulation of acute inflammatory response to non-antigenic stimulus; glucose homeostasis; leukocyte chemotaxis during inflammatory response; positive regulation of chemokine production