Ferrous iron transport protein B (feoB) Recombinant Protein | feoB recombinant protein

Recombinant Escherichia coli Ferrous iron transport protein B (feoB), partial

Cat. Num. AAA1163875

• Gene Names: feoB; ECK3396; JW3372
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ferrous iron transport protein B (feoB); Recombinant Escherichia coli Ferrous iron transport protein B (feoB); partial; feoB recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-773
• Sequence: MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQFSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNLYLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALKLAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAYAGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDKIVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDWLTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGKSFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQNGALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRLKGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHEDNWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSLKDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMGAIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIGLLRRARSRVDIELLATRKSVSSCCAASTTGDCH
• Sequence Length: 773

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16131285
• NCBI GeneID: 947919
• NCBI Accession #: NP_417868.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P33650; Q2M774; Q6IU42
• Molecular Weight: 84,474 Da
• NCBI Official Full Name: ferrous iron transporter protein B and GTP-binding protein; membrane protein
• NCBI Official Symbol: feoB
• NCBI Official Synonym Symbols: ECK3396; JW3372
• NCBI Protein Information: ferrous iron transporter protein B and GTP-binding protein; membrane protein
• UniProt Protein Name: Fe(2+) transporter FeoB
• Protein Family: Ferrous iron transport protein
• UniProt Gene Name: feoB

NCBI Description

FeoB GTPase actvity is required for ferrous iron uptake; it slowly hydrolyzes its bound GTP. [More information is available at EcoGene: EG12102]. FeoB is a ferrous iron uptake system belonging to the Ferrous Iron Uptake (FeoB) transporter family . [More information is available at EcoCyc: EG12102].

Uniprot Description

Transporter of a GTP-driven Fe2+ uptake system, probably couples GTP-binding to channel opening and Fe2+ uptake (PubMed:12446835, PubMed:19629046). A guanine nucleotide-binding protein (G proteins) in which the guanine nucleotide binding site alternates between an active, GTP-bound state and an inactive, GDP-bound state. This protein has fast intrinsic GDP release, mediated by the G5 loop (about residues 149-158). Presumably GTP hydrolysis leads to conformational changes and channel closing (PubMed:19629046). A GDP release mechanism involving a conformational change of the G5 loop (and thus the removal of the nucleotide-binding and stabilizing interactions) would significantly reduce the affinity for GDP, and conceivably be sufficient for catalysing nucleotide release (PubMed:25374115).