Rat flap structure-specific endonuclease 1 ELISA Kit | FEN1 elisa kit
Rat Flap endonuclease 1, FEN1 ELISA Kit
NCBI and Uniprot Product Information
NCBI Description
removes RNA primers during Okazaki fragment maturation in DNA synthesis [RGD, Feb 2006]
Uniprot Description
FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Protein type: DNA replication; Enzyme, misc.; DNA-binding; Ribonuclease; Hydrolase; Deoxyribonuclease; Mitochondrial; DNA repair, damage; Nuclear receptor co-regulator; EC 3.1.-.-; Nucleolus
Cellular Component: nucleoplasm; membrane; mitochondrion; plasma membrane; nucleolus; nucleus
Molecular Function: 5'-3' exonuclease activity; DNA binding; 5'-flap endonuclease activity; manganese ion binding; exonuclease activity; magnesium ion binding; ribonuclease H activity; flap endonuclease activity
Biological Process: DNA replication, removal of RNA primer; base-excision repair; DNA repair; DNA replication; DNA catabolic process, endonucleolytic; memory
Research Articles on FEN1
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Product Notes
The Rat FEN1 fen1 (Catalog #AAA9339023) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9339023 ELISA Kit recognizes Rat FEN1. It is sometimes possible for the material contained within the vial of "flap structure-specific endonuclease 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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