Ferredoxin Reductase (FDXR) Recombinant Protein | FDXR recombinant protein

Recombinant Ferredoxin Reductase (FDXR)

Cat. Num. AAA2086910

• Gene Names: FDXR; ADXR
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Ferredoxin Reductase (FDXR); Recombinant Ferredoxin Reductase (FDXR); ADXR; AR; Adrenodoxin Oxidoreductase; Mitochondrial; Adrenodoxin reductase; Ferredoxin--NADP(+) reductase; FDXR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 534

• Applicable Applications for FDXR recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ser33~Arg219
• Tag: N-terminal His-tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 5.3
• Subcellular Location: Mitochondrion
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Product Categories/Family for FDXR recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 731184190
• NCBI GeneID: 2232
• NCBI Accession #: NP_001244941.2
• NCBI GenBank Nucleotide #: NM_001258012.3
• UniProt Accession #: P22570; Q13716; Q4PJI0; Q9BU12; B4DDI7; B4DHX5; B4DQQ4; B4DX24; B7Z7G2; E7EQC1
• Molecular Weight: 24.1kDa
• NCBI Official Full Name: NADPH:adrenodoxin oxidoreductase, mitochondrial isoform 3
• NCBI Official Synonym Full Names: ferredoxin reductase
• NCBI Official Symbol: FDXR
• NCBI Official Synonym Symbols: ADXR
• NCBI Protein Information: NADPH:adrenodoxin oxidoreductase, mitochondrial
• UniProt Protein Name: NADPH:adrenodoxin oxidoreductase, mitochondrial
• UniProt Gene Name: FDXR
• UniProt Synonym Gene Names: ADXR; AR; Adrenodoxin reductase; Ferredoxin reductase

NCBI Description

This gene encodes a mitochondrial flavoprotein that initiates electron transport for cytochromes P450 receiving electrons from NADPH. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

FDXR: Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. Belongs to the ferredoxin--NADP reductase type 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.18.1.6; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: ferredoxin-NADP+ reductase activity; NADPH-adrenodoxin reductase activity; protein binding

Biological Process: C21-steroid hormone biosynthetic process; generation of precursor metabolites and energy; steroid biosynthetic process; sterol metabolic process