SDS-Page
Ferredoxin 1 Recombinant Protein | FDX1 recombinant protein
Ferredoxin 1, 61-184aa, Human, T7-tag, E Coli
Gene Names
FDX1; ADX; FDX; LOH11CR1D
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Ferredoxin 1; 61-184aa; Human; T7-tag; E Coli; Adrenodoxin; Adrenal ferredoxin; Hepatoredoxin; ADX; FDX; LOH11CR1D; mitochondrial; FDX1; ; FDX1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS
Sequence Length
184
Applicable Applications for FDX1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-T7 tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for FDX1 recombinant protein
Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for FDX1 recombinant protein
References
Mandai T., et al. (2009). FEBS J. 276 (8):2416-29. ; Pichiorri F., et al. (2009). J Biol Chem. 284 (2):1040-9.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.0 kDa (139aa), confirmed by MALDI-TOF.
NCBI Official Full Name
adrenodoxin, mitochondrial
NCBI Official Synonym Full Names
ferredoxin 1
NCBI Official Symbol
FDX1
NCBI Official Synonym Symbols
ADX; FDX; LOH11CR1D
NCBI Protein Information
adrenodoxin, mitochondrial
UniProt Protein Name
Adrenodoxin, mitochondrial
Protein Family
UniProt Gene Name
FDX1
UniProt Synonym Gene Names
ADX
NCBI Description
This gene encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism. Pseudogenes of this functional gene are found on chromosomes 20 and 21. [provided by RefSeq, Aug 2011]
Uniprot Description
Participates in the synthesis of thyroid hormones. Essential for the synthesis of various steroid hormones, participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage.
Research Articles on FDX1
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Product Notes
The FDX1 fdx1 (Catalog #AAA203360) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-T7 tag. AAA Biotech's Ferredoxin 1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the FDX1 fdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS. It is sometimes possible for the material contained within the vial of "Ferredoxin 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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