Human fructose-1, 6-bisphosphatase 2 ELISA Kit | FBP2 elisa kit

Human Fructose-1, 6-bisphosphatase isozyme 2, FBP2 ELISA Kit

Cat. Num. AAA9323366

• Reactivity: Human
• Synonyms: fructose-1; 6-bisphosphatase 2; Human Fructose-1; 6-bisphosphatase isozyme 2; FBP2 ELISA Kit; 6-bisphosphatase isozyme 2 (FBP2) ELISA kit; MGC142192; D-fructose-1; 6-bisphosphate 1-phosphohydrolase; FBPase; hexosediphosphatase; muscle fructose-bisphosphatase; FBP2 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Body fluids, tissue homogenates, secretions or feces samples
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml
• Sensitivity: 0.1 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FBP2 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of FBP2 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 22907028
• NCBI GeneID: 8789
• NCBI Accession #: NP_003828.2
• NCBI GenBank Nucleotide #: NM_003837.3
• UniProt Accession #: O00757; Q17R39; Q6FI53
• Molecular Weight: 36,743 Da
• NCBI Official Full Name: fructose-1,6-bisphosphatase isozyme 2
• NCBI Official Synonym Full Names: fructose-1,6-bisphosphatase 2
• NCBI Official Symbol: FBP2
• NCBI Protein Information: fructose-1,6-bisphosphatase isozyme 2; FBPase 2; muscle FBPase; hexosediphosphatase; muscle fructose-bisphosphatase; D-fructose-1,6-bisphosphate 1-phosphohydrolase 2
• UniProt Protein Name: Fructose-1,6-bisphosphatase isozyme 2
• Protein Family: Fat body protein
• UniProt Gene Name: FBP2
• UniProt Synonym Gene Names: FBPase 2
• UniProt Entry Name: F16P2_HUMAN

NCBI Description

This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]

Uniprot Description

FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.

Protein type: EC 3.1.3.11; Carbohydrate Metabolism - pentose phosphate pathway; Phosphatase (non-protein); Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cell junction; Z disc; nucleus; cytosol

Molecular Function: metal ion binding; fructose-bisphosphatase activity

Biological Process: dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose metabolic process; gluconeogenesis