Monkey Fibrinogen ELISA Kit | FBG elisa kit

Monkey Fibrinogen ELISA Kit

Cat. Num. AAA22981

• Reactivity: Monkey
• Synonyms: Fibrinogen; Monkey Fibrinogen ELISA Kit; FBG elisa kit

• Reactivity: Monkey
• Specificity: No significant cross-reactivity or interference between Monkey FBG and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.25 mg/ml - 8 mg/ml.
• Sensitivity: 0.1 mg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated FBG concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey FBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FBG and FBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for FBG elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated FBG concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey FBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FBG and FBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 350
• NCBI GeneID: 280792
• UniProt Accession #: P12799
• Molecular Weight: 50,244 Da
• NCBI Official Full Name: fibrinogen
• NCBI Official Synonym Full Names: fibrinogen gamma chain
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide
• UniProt Protein Name: Fibrinogen gamma-B chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Synonym Gene Names: Gamma'
• UniProt Entry Name: FIBG_BOVIN

Uniprot Description

Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Subunit structure: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain

By similarity.

Subcellular location: Secreted.

Domain: A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

Post-translational modification: Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.