SDS-PAGE
3-oxoacyl-[acyl-carrier-protein] synthase 2 Recombinant Protein | fabF recombinant protein
Recombinant Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2
Gene Names
fabF; cvc; ECK1081; fabJ; JW1081; vtr
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
3-oxoacyl-[acyl-carrier-protein] synthase 2; Recombinant Escherichia coli (strain K12) 3-oxoacyl-[acyl-carrier-protein] synthase 2; 3-oxoacyl-[acyl-carrier-protein] synthase II; Beta-ketoacyl-ACP synthase II; KAS II; fabF recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
2-413aa; Full Length
Sequence
SKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTSAYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIGAAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISIATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPENGAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSSTKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGMEYTLCNSFGFGGTNGSLIFKKI
Sequence Length
413
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for fabF recombinant protein
Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.
References
The fabJ-encoded beta-ketoacyl- synthase IV from Escherichia coli is sensitive to cerulenin and specific for short-chain substrates.Siggaard-Andersen M., Wissenbach M., Chuck J.-A., Svendsen I., Olsen J.G., von Wettstein-Knowles P.V.Proc. Natl. Acad. Sci. U.S.A. 91:11027-11031(1994)
The putative fabJ gene of Escherichia coli fatty acid synthesis is the fabF gene.Magnuson K., Carey M.R., Cronan J.E. Jr.J. Bacteriol. 177:3593-3595(1995)
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF)
mutants.Jackowski S., Rock C.O.J. Bacteriol. 169:1469-1473(1987)
Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis.Garwin J.L., Klages A.L., Cronan J.E. Jr.J. Biol. Chem. 255:3263-3265(1980)
Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli.Edwards P., Nelsen J.S., Metz J.G., Dehesh K.FEBS Lett. 402:62-66(1997)
Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E Coli reveals the molecular architecture of condensing enzymes.Huang W., Jia J., Edwards P., Dehesh K., Schneider G., Lindqvist Y.EMBO J. 17:1183-1191(1998)
Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase.Moche M., Schneider G., Edwards P., Dehesh K., Lindqvist Y.J. Biol. Chem. 274:6031-6034(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58.9 kDa
NCBI Official Full Name
3-oxoacyl-[acyl-carrier-protein] synthase II
NCBI Official Symbol
fabF
NCBI Official Synonym Symbols
cvc; ECK1081; fabJ; JW1081; vtr
NCBI Protein Information
3-oxoacyl-[acyl-carrier-protein] synthase II
UniProt Protein Name
3-oxoacyl-[acyl-carrier-protein] synthase 2
Protein Family
UniProt Gene Name
fabF
UniProt Synonym Gene Names
fabJ; KAS II
UniProt Entry Name
FABF_ECOLI
NCBI Description
There are three -ketoacyl-ACP synthases (KAS) in Escherichia coli: |FRAME: [More information is available at EcoCyc: EG12606].
Uniprot Description
Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis.
Research Articles on fabF
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Product Notes
The fabF fabf (Catalog #AAA1166991) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-413aa; Full Length. The amino acid sequence is listed below: SKRRVVVTGL GMLSPVGNTV ESTWKALLAG QSGISLIDHF DTSAYATKFA GLVKDFNCED IISRKEQRKM DAFIQYGIVA GVQAMQDSGL EITEENATRI GAAIGSGIGG LGLIEENHTS LMNGGPRKIS PFFVPSTIVN MVAGHLTIMY GLRGPSISIA TACTSGVHNI GHAARIIAYG DADVMVAGGA EKASTPLGVG GFGAARALST RNDNPQAASR PWDKERDGFV LGDGAGMLVL EEYEHAKKRG AKIYAELVGF GMSSDAYHMT SPPENGAGAA LAMANALRDA GIEASQIGYV NAHGTSTPAG DKAEAQAVKT IFGEAASRVL VSSTKSMTGH LLGAAGAVES IYSILALRDQ AVPPTINLDN PDEGCDLDFV PHEARQVSGM EYTLCNSFGF GGTNGSLIFK KI. It is sometimes possible for the material contained within the vial of "3-oxoacyl-[acyl-carrier-protein] synthase 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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