Monkey Coagulation Factor VII ELISA Kit | F7 elisa kit

Monkey Coagulation Factor VII ELISA Kit

Cat. Num. AAA739575

• Gene Names: F7; SPCA
• Reactivity: Monkey
• Synonyms: Coagulation Factor VII; Monkey Coagulation Factor VII ELISA Kit; F7 elisa kit

• Reactivity: Monkey
• Specificity: This assay has high sensitivity and excellent specificity for detection of FVII. No significant cross-reactivity or interference between FVII and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FVII and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for F7 elisa kit

Intended Uses: This FVII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey FVII. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: FVII ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FVII antibody and an FVII-HRP conjugate. The assay sample and buffer are incubated together with FVII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FVII concentration since FVII from samples and FVII-HRP conjugate compete for the anti-FVII antibody binding site. Since the number of sites is limited, as more sites are occupied by FVII from the sample, fewer sites are left to bind FVII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FVII concentration in each sample is interpolated from this standard curve.

Product Categories/Family for F7 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 12750767
• NCBI GeneID: 2155
• Molecular Weight: 49,320 Da
• NCBI Official Full Name: coagulation factor VII, partial
• NCBI Official Synonym Full Names: coagulation factor VII (serum prothrombin conversion accelerator)
• NCBI Official Symbol: F7
• NCBI Official Synonym Symbols: SPCA
• NCBI Protein Information: coagulation factor VII; eptacog alfa; proconvertin; FVII coagulation protein
• UniProt Protein Name: Coagulation factor VII
• Protein Family: Coagulation factor
• UniProt Gene Name: F7
• UniProt Synonym Gene Names: SPCA
• UniProt Entry Name: FA7_HUMAN

NCBI Description

This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

F7: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. Defects in F7 are the cause of factor VII deficiency (FA7D). A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.21; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region; plasma membrane; vesicle

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; calcium ion binding; glycoprotein binding; receptor binding

Biological Process: circadian rhythm; organ regeneration; positive regulation of blood coagulation; positive regulation of positive chemotaxis; positive regulation of leukocyte chemotaxis; post-translational protein modification; proteolysis; peptidyl-glutamic acid carboxylation; positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; response to vitamin K; response to estrogen stimulus; blood coagulation; positive regulation of cell migration

Disease: Myocardial Infarction, Susceptibility To; Factor Vii Deficiency