Mouse Coagulation Factor 5 Antigen ELISA Kit | F5-Ag elisa kit

Mouse Coagulation Factor 5 Antigen ELISA Kit

Cat. Num. AAA729369

• Gene Names: F5; FVL; PCCF; THPH2; RPRGL1
• Reactivity: Mouse
• Synonyms: Coagulation Factor 5 Antigen; Mouse Coagulation Factor 5 Antigen ELISA Kit; F5-Ag elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This F5-AG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse F5-AG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F5-Ag elisa kit

Principle of the Assay: F5-AG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F5-AG antibody and an F5-AG-HRP conjugate. The assay sample and buffer are incubated together with F5-AG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F5-AG concentration since F5-AG from samples and F5-AG-HRP conjugate compete for the anti-F5-AG antibody binding site. Since the number of sites is limited, as more sites are occupied by F5-AG from the sample, fewer sites are left to bind F5-AG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F5-AG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for F5-Ag elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 105990535
• NCBI GeneID: 2153
• NCBI Accession #: NP_000121.2
• NCBI GenBank Nucleotide #: NM_000130.4
• Molecular Weight: 251,703 Da
• NCBI Official Full Name: coagulation factor V
• NCBI Official Synonym Full Names: coagulation factor V (proaccelerin, labile factor)
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: FVL; PCCF; THPH2; RPRGL1
• NCBI Protein Information: coagulation factor V; factor V Leiden; proaccelerin, labile factor; activated protein c cofactor; coagulation factor V jinjiang A2 domain
• UniProt Protein Name: Coagulation factor V
• UniProt Gene Name: F5
• UniProt Entry Name: FA5_HUMAN

NCBI Description

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

Uniprot Description

factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Secreted, signal peptide; Secreted; Protease

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; plasma membrane; extracellular region; vesicle

Molecular Function: protein binding; copper ion binding; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; blood circulation; proteolysis; blood coagulation

Disease: Thrombophilia Due To Activated Protein C Resistance; Budd-chiari Syndrome; Pregnancy Loss, Recurrent, Susceptibility To, 1; Stroke, Ischemic; Factor V Deficiency