Protease Activated Receptor 2 Recombinant Protein | PAR2 recombinant protein

Recombinant Protease Activated Receptor 2 (PAR2)

Cat. Num. AAA2122721

• Gene Names: F2rl1; Par2; Par-2
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Protease Activated Receptor 2; Recombinant Protease Activated Receptor 2 (PAR2); F2RL1; F2-RL1; GPR11; PAR2; Coagulation Factor II(thrombin)receptor-Like 1; G-protein coupled receptor 11; Thrombin receptor-like 1; PAR2 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Val309~Tyr397
• Sequence Length: 397

• Applicable Applications for PAR2 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Rat
• Tag: N-terminal His and GST Tag
• Subcellular Location: Membrane
• Isoelectric Point: 10
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for PAR2 recombinant protein

Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 16758770
• NCBI GeneID: 116677
• NCBI Accession #: NP_446349.1
• NCBI GenBank Nucleotide #: NP_446349.1
• UniProt Accession #: Q63645
• Molecular Weight: Predicted Molecular Mass: 40kDa; Accurate Molecular Mass: 42kDa
• NCBI Official Full Name: proteinase-activated receptor 2
• NCBI Official Synonym Full Names: F2R like trypsin receptor 1
• NCBI Official Symbol: F2rl1
• NCBI Official Synonym Symbols: Par2; Par-2
• NCBI Protein Information: proteinase-activated receptor 2
• UniProt Protein Name: Proteinase-activated receptor 2
• Protein Family: Transcription factor
• UniProt Gene Name: F2rl1
• UniProt Synonym Gene Names: Par2; PAR-2

NCBI Description

G-coupled protein receptor that is activated by coagulation factor II and other serine proteases; may play a role in regulation of exocrine secretion [RGD, Feb 2006]

Uniprot Description

Receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Can also be transactivated by cleaved F2r/Par1. Involved in modulation of inflammatory responses and regulation of innate and adaptive immunity, and acts as a sensor for proteolytic enzymes generated during infection. Generally is promoting inflammation. Can signal synergistically with Tlr4 and probably Tlr2 in inflammatory responses and modulates Tlr3 signaling. Has a protective role in establishing the endothelial barrier; the activity involves coagulation factor X. Proposed to have a bronchoprotective role in airway epithelium, but also shown to compromise the airway epithelial barrier by interrupting E-cadherin adhesion. Involved in the regulation of vascular tone; activation results in hypotension presumably mediated by vasodilation. Associates with a subset of G proteins alpha subunits such as G alpha-q, G alpha-11, G alpha-14, G alpha-12 and G alpha-13, but probably not with G(o) alpha, G(i) subunit alpha-1 and G(i) subunit alpha-2. Believed to be a class B receptor which internalizes as a complex with arrestin and traffic with it to endosomal vesicles, presumably as desensitized receptor, for extended periods of time. Mediates inhibition of TNF-alpha stimulated JNK phosphorylation via coupling to G alpha-q/11; the function involves dissociation of Ripk1 and Tradd from Tnfr1. Mediates phosphorylation of nuclear factor NF-kappa-B RELA subunit at 'Ser-536'; the function involves Ikbkb and is predominantly independent of G proteins. Involved in cellular migration. Involved in cytoskeletal rearrangement and chemotaxis through beta-arrestin-promoted scaffolds; the function is independent of G alpha-q/11 and involves promotion of cofilin dephosphoryltaion and actin filament severing. Induces redistribution of Cops5 from the plasma membrane to the cytosol and activation of the JNK cascade is mediated by Cops5. Involved in the recruitment of leukocytes to the sites of inflammation and is the major PAR receptor capable of modulating eosinophil function such as proinflammatory cytokine secretion, superoxide production and degranulation. During inflammation promotes dendritic cell maturation, trafficking to the lymph nodes and subsequent T-cell activation. Involved in antimicrobial response of innate immnune cells; activation enhances phagocytosis of Gram-positive and killing of Gram-negative bacteria. Acts synergistically with interferon-gamma in enhancing antiviral responses ().