Mouse Coagulation Factor XIII B Polypeptide ELISA Kit | F13B elisa kit

Mouse Coagulation Factor XIII B Polypeptide ELISA Kit

Cat. Num. AAA726067

• Gene Names: F13B; FXIIIB
• Reactivity: Mouse
• Synonyms: Coagulation Factor XIII B Polypeptide; Mouse Coagulation Factor XIII B Polypeptide ELISA Kit; F13B elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This F13B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse F13B. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F13B elisa kit

Principle of the Assay: F13B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F13B antibody and an F13B-HRP conjugate. The assay sample and buffer are incubated together with F13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F13B concentration since F13B from samples and F13B-HRP conjugate compete for the anti-F13B antibody binding site. Since the number of sites is limited, as more sites are occupied by F13B from the sample, fewer sites are left to bind F13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F13B concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110611237
• NCBI GeneID: 2165
• NCBI Accession #: NP_001985.2
• NCBI GenBank Nucleotide #: NM_001994.2
• Molecular Weight: 75,511 Da
• NCBI Official Full Name: coagulation factor XIII B chain
• NCBI Official Synonym Full Names: coagulation factor XIII, B polypeptide
• NCBI Official Symbol: F13B
• NCBI Official Synonym Symbols: FXIIIB
• NCBI Protein Information: coagulation factor XIII B chain; TGase; transglutaminase B chain; fibrin-stabilizing factor B subunit; protein-glutamine gamma-glutamyltransferase B chain
• UniProt Protein Name: Coagulation factor XIII B chain
• Protein Family: Coagulation factor
• UniProt Gene Name: F13B
• UniProt Entry Name: F13B_HUMAN

NCBI Description

This gene encodes coagulation factor XIII B subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

F13B: The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin. Defects in F13B are the cause of factor XIII subunit B deficiency (FA13BD). FA13BD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q31-q32.1

Cellular Component: extracellular region

Biological Process: blood coagulation

Disease: Factor Xiii, B Subunit, Deficiency Of