Typical Testing Data/Standard Curve (for reference only)
Monkey Coagulation Factor X ELISA Kit | F10 elisa kit
Monkey Coagulation Factor X ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: F10 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-F10 antibody and an F10-HRP conjugate. The assay sample and buffer are incubated together with F10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F10 concentration since F10 from samples and F10-HRP conjugate compete for the anti-F10 antibody binding site. Since the number of sites is limited, as more sites are occupied by F10 from the sample, fewer sites are left to bind F10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F10 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008]
Uniprot Description
F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.
Protein type: Lipid-binding; Secreted, signal peptide; EC 3.4.21.6; Protease; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane
Molecular Function: protein binding; serine-type endopeptidase activity; phospholipid binding; calcium ion binding
Biological Process: positive regulation of protein kinase B signaling cascade; blood coagulation, extrinsic pathway; cellular protein metabolic process; blood coagulation; post-translational protein modification; proteolysis; peptidyl-glutamic acid carboxylation; blood coagulation, intrinsic pathway; positive regulation of cell migration
Disease: Factor X Deficiency
Research Articles on F10
Similar Products
Product Notes
The Monkey F10 f10 (Catalog #AAA735040) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735040 ELISA Kit recognizes Monkey F10. It is sometimes possible for the material contained within the vial of "Coagulation Factor X, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
