Viral RNA Extraction Kit

Viral RNA Extraction kit (Respiratory Specimens)

Applications

ELISA

Synonyms

Viral RNA; Viral RNA Extraction kit (Respiratory Specimens); Viral RNA extraction kit

Ordering
Cat. Num. AAA669457 For Research Use Only!

Applicable Applications for Viral RNA extraction kit

ELISA (EIA)

Application Notes

The protocol is developed for 200-300 uL samples. Small samples should be adjusted to 200-300 uL with phosphate-buffered saline (PBS) before loading. Pipet 20 uL Proteinase K, 2 uL L Solution, and 300 uL Buffer LYE to a 1.5 mL tube. Calculate the number of samples to be processed and make master mix of proteinase K, L Solution and Buffer LYE. Pipet 300 uL nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, or plasma, serum, into the 1.5 mL tube from Step 1. Mix well by pulse-vortexting for 10 seconds. Spin briefly to collect the drops from the lid. Incubate at room temperature for 10 min to lyse the cells and virus. Add 600 uL isopropanol and mix well by pulse-vortexing for 5 seconds. Spin briefly to collect the drop from the lid. Transfer 600 uL of the sample from step 3 into a RNA column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through carefully to a waste container by pipetting and put the column back to the collection Transfer the remaining sample to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the collection tube and transfer the RNA column to a new collection tube. Add 500 uL Buffer RB to the column and centrifuge at 10,000 rm for 30 seconds. Discard the flow-through. Put the column back to the collection tube. Add 500 uL RNA Wash Buffer to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through. Put the column back to the collection tube. Centrifuge the empty column at 12,000 rpm for 2 min. It is critical to remove residual ethanol for optimal elution. Transfer the RNA column to a RNase-free 5 mL tube, add 35-50 uL DEPC-treated water to the column and centrifuge at 10,000 rpm for 30 seconds. The viral RNA is in the flow-through liquid. Optional: Add the eluent back to the column for a second elution. Note: The first elution normally yields 70% of the RNA while the second elution yields another 20-30% of the RNA bound to the column. Note: The purified RNA should be put on ice for downstream application or store at -20 degree C.

Preparation and Storage

All components can be stored at room temperature (15-25 degree C). All kit components are guaranteed for 1 year from the date of purchase.

Related Product Information for Viral RNA extraction kit

The Viral DNA/RNA mini kit provides an easy and reliable method for isolating total viral RNA from plasma, serum, nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. This procedure has been tested for isolating nucleic acids from COVID-19, Hepatitis A, Hepatitis C and HIV. The isolated RNA can be used for PCR, qRT-PCR and other downstream applications.
Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable.

Product Categories/Family for Viral RNA extraction kit

Kits and Reagents; Molecular Biology Kits

Similar Products

Product Notes

The Viral RNA (Catalog #AAA669457) is an Extraction Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Viral RNA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The protocol is developed for 200-300 uL samples. Small samples should be adjusted to 200-300 uL with phosphate-buffered saline (PBS) before loading. Pipet 20 uL Proteinase K, 2 uL L Solution, and 300 uL Buffer LYE to a 1.5 mL tube. Calculate the number of samples to be processed and make master mix of proteinase K, L Solution and Buffer LYE. Pipet 300 uL nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, or plasma, serum, into the 1.5 mL tube from Step 1. Mix well by pulse-vortexting for 10 seconds. Spin briefly to collect the drops from the lid. Incubate at room temperature for 10 min to lyse the cells and virus. Add 600 uL isopropanol and mix well by pulse-vortexing for 5 seconds. Spin briefly to collect the drop from the lid. Transfer 600 uL of the sample from step 3 into a RNA column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through carefully to a waste container by pipetting and put the column back to the collection Transfer the remaining sample to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the collection tube and transfer the RNA column to a new collection tube. Add 500 uL Buffer RB to the column and centrifuge at 10,000 rm for 30 seconds. Discard the flow-through. Put the column back to the collection tube. Add 500 uL RNA Wash Buffer to the column and centrifuge at 10,000 rpm for 30 seconds. Discard the flow-through. Put the column back to the collection tube. Centrifuge the empty column at 12,000 rpm for 2 min. It is critical to remove residual ethanol for optimal elution. Transfer the RNA column to a RNase-free 5 mL tube, add 35-50 uL DEPC-treated water to the column and centrifuge at 10,000 rpm for 30 seconds. The viral RNA is in the flow-through liquid. Optional: Add the eluent back to the column for a second elution. Note: The first elution normally yields 70% of the RNA while the second elution yields another 20-30% of the RNA bound to the column. Note: The purified RNA should be put on ice for downstream application or store at -20 degree C. Researchers should empirically determine the suitability of the Viral RNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Viral RNA, Extraction Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Viral RNA Extraction Kit