Vascular Endothelial Growth Factor A (VEGFA) Active Protein | VEGFA active protein

Active Vascular Endothelial Growth Factor A (VEGFA)

Cat. Num. AAA146617

• Gene Names: Vegfa; VPF; Vegf; VEGF-A; VEGF111; VEGF164
• Applications: Activity Assay, Cell Culture
• Purity: > 97%
• Synonyms: Vascular Endothelial Growth Factor A (VEGFA); N/A; Active Vascular Endothelial Growth Factor A (VEGFA); VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor; VEGFA active protein

• Host: 293F cell
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 325

• Applicable Applications for VEGFA active protein: Activity Assay, Cell Culture
• Fragment: Ala27~Arg190
• Tag: N-terminal His Tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Organism Species: Rat
• Isoelectric Point: 7.9
• Usage: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

Application Data

SDS-PAGE

Activity

(Vascular endothelial growth factor A (VEGF-A), a glycosylated mitogen, is known to be a vascular permeability factor and an endothelial cell growth factor secreted by the smooth muscle and endothelial cells. It has been reported that VEGF-A induces vascular permeability and growth, promotes monocyte/macrophage migration, and inhibits cell apoptosis and so on. To test the effect of VEGF-A on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFA. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC .Cell proliferation of ECV304 cells after incubation with VEGFA for 72h observed by inverted microscope was shown in Figure 1.)

Product Categories/Family for VEGFA active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 160358797
• NCBI GeneID: 83785
• NCBI Accession #: NP_001103804.1
• NCBI GenBank Nucleotide #: NM_001110334.2
• UniProt Accession #: P16612; Q541S6; Q91ZE1; Q9JKX7; Q9QXG6; Q9QXG7
• Molecular Weight: 20.8kDa; 22kDa
• NCBI Official Full Name: vascular endothelial growth factor A isoform 3
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: Vegfa
• NCBI Official Synonym Symbols: VPF; Vegf; VEGF-A; VEGF111; VEGF164
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: Vegfa
• UniProt Synonym Gene Names: Vegf; VEGF-A; VPF