Eukaryotes Paraoxonase 1 (PON1) Assay Kit | PON1 assay kit

Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric)

Cat. Num. AAA846929

• Gene Names: PON1; ESA; PON; MVCD5
• Reactivity: Eukaryotes
• Synonyms: Paraoxonase 1 (PON1); Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric); PON1 assay kit

• Reactivity: Eukaryotes
• Sequence Length: 6

• Application Notes: Rapid assessment of native/recombinant Paraoxonase; - Paraoxonase activity in mammalian serum and plasm
• Preparation and Storage: Store at -20 degree C for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PON1 assay kit

Background/Introduction: Paraoxonase 1 (PON1, EC 3.1.8.1) is a 43 kDa enzyme synthesized in the liver and released into the bloodstream, where it associates with high-density lipoprotein (HDL) particles in serum. PON1 is a promiscuous enzyme with broad-spectrum hydrolase activity that was originally named for its ability to hydrolyze highly toxic organophosphorus compounds such as the insecticide paraoxon and various nerve agents used as chemical weapons. The enzyme has subsequently been demonstrated to catalyze hydrolysis of lipid hydroperoxides and lactones. PON1 helps protect serum HDL and LDL particles against lipid peroxidation and inhibits N-homocysteinylation of LDLassociated proteins by hydrolyzing the highly reactive pro-oxidant homocysteine thiolactone. Accumulation of lipid peroxides and homocysteinylated proteins triggers arterial inflammation and eventually leads to atherosclerosis, ischemic stroke and myocardial infarction. PON1 activity is considered to be a clinical biomarker of hepatic and systemic oxidative stress. Measurement of serum PON1 activity has been proposed as a potential test for evaluation of liver function and risk of cardiovascular disease.. Paraoxonase 1 Activity Assay Kit enables rapid measurement of PON1 activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 uU paraoxonase activity with a sample volume of 5 ul.

Product Categories/Family for PON1 assay kit

Oxidative Stress

NCBI and Uniprot Product Information

• NCBI GI #: 345894407
• NCBI GeneID: 5444
• Molecular Weight: 39,731 Da
• NCBI Official Full Name: paraoxonase 1, partial
• NCBI Official Synonym Full Names: paraoxonase 1
• NCBI Official Symbol: PON1
• NCBI Official Synonym Symbols: ESA; PON; MVCD5
• NCBI Protein Information: serum paraoxonase/arylesterase 1
• UniProt Protein Name: Serum paraoxonase/arylesterase 1
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON1
• UniProt Synonym Gene Names: PON; A-esterase 1

NCBI Description

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

Uniprot Description

PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; Hydrolase; Lipid-binding; Motility/polarity/chemotaxis; Phosphatase (non-protein); Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular region; extracellular space

Molecular Function: aryldialkylphosphatase activity; arylesterase activity; calcium ion binding; phospholipid binding; protein homodimerization activity

Biological Process: aromatic compound catabolic process; carboxylic acid catabolic process; lipoxygenase pathway; organophosphate catabolic process; phosphatidylcholine metabolic process; positive regulation of binding; positive regulation of transporter activity; response to toxin

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5