Human Electron transfer flavoprotein subunit beta ELISA Kit | ETFbeta elisa kit

Human Electron transfer flavoprotein subunit beta ELISA Kit

Cat. Num. AAA761519

• Gene Names: ETFB; MADD; FP585
• Reactivity: Human
• Synonyms: Electron transfer flavoprotein subunit beta; Human Electron transfer flavoprotein subunit beta ELISA Kit; ETFb (Electron transfer flavoprotein subunit beta)/FP585/Beta-ETF; ETFbeta elisa kit

• Reactivity: Human
• Sequence Length: 346

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ETFbeta elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ETFbeta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ETFbeta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ETFbeta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ETFbeta can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62420877
• NCBI GeneID: 2109
• NCBI Accession #: NP_001014763.1
• NCBI GenBank Nucleotide #: NM_001014763.1
• Molecular Weight: 37,434 Da
• NCBI Official Full Name: electron transfer flavoprotein subunit beta isoform 2
• NCBI Official Synonym Full Names: electron transfer flavoprotein beta subunit
• NCBI Official Symbol: ETFB
• NCBI Official Synonym Symbols: MADD; FP585
• NCBI Protein Information: electron transfer flavoprotein subunit beta
• UniProt Protein Name: Electron transfer flavoprotein subunit beta
• Protein Family: Electron transfer flavoprotein
• UniProt Gene Name: ETFB
• UniProt Synonym Gene Names: Beta-ETF
• UniProt Entry Name: ETFB_HUMAN

NCBI Description

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ETFB: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B). GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF beta-subunit/FixA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion

Molecular Function: electron carrier activity

Biological Process: cellular metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase

Disease: Multiple Acyl-coa Dehydrogenase Deficiency