Human Endogenous Secretory Receptor For Advanced Glycation End Products ELISA Kit | ESRAGE elisa kit

Human Endogenous Secretory Receptor For Advanced Glycation End Products ELISA Kit

Cat. Num. AAA15358

• Gene Names: Ager; RAGE
• Reactivity: Human
• Synonyms: Endogenous Secretory Receptor For Advanced Glycation End Products; Human Endogenous Secretory Receptor For Advanced Glycation End Products ELISA Kit; ESRAGE elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human ESRAGE and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ESRAGE concentrations in Human serum, plasma and other body fluids. Using Purified Human ESRAGE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ESRAGE and ESRAGE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ESRAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ESRAGE elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ESRAGE concentrations in Human serum, plasma and other body fluids. Using Purified Human ESRAGE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ESRAGE and ESRAGE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ESRAGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 84570471
• NCBI GeneID: 11596
• UniProt Accession #: Q62151
• Molecular Weight: 42,669 Da
• NCBI Official Full Name: endogenous secretory receptor for advanced glycation endproducts
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor; advanced glycation end-products receptor; receptor for advanced glycosylation end products
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_MOUSE

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Cell cycle regulation; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell surface; membrane; cell soma; axon; cytoplasm; integral to membrane; basal plasma membrane; plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; advanced glycation end-product receptor activity

Biological Process: positive regulation of JNK activity; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; negative regulation of cell adhesion; positive regulation of smooth muscle cell migration; JAK-STAT cascade; induction of positive chemotaxis; activation of NF-kappaB transcription factor; calcium ion homeostasis; positive regulation of fibroblast proliferation; negative regulation of protein amino acid phosphorylation; regulation of DNA binding; positive regulation of neuron apoptosis; negative regulation of endothelial cell proliferation; regulation of inflammatory response; response to hypoxia; positive regulation of protein amino acid phosphorylation; inflammatory response; positive regulation of phagocytosis, engulfment; positive regulation of autophagy; neurite development; positive regulation of inflammatory response; positive regulation of cell migration