L-Asparaginase Enzyme | ansA enzyme
L-Asparaginase (Amidohydrolase)
Purity
> 96% by RP-HPLC and SDS-PAGE
Synonyms
L-Asparaginase; L-Asparaginase (Amidohydrolase); ansA enzyme
Host
Escherichia Coli.
Purity/Purification
> 96% by RP-HPLC and SDS-PAGE
Form/Format
Supplied as a lyophilized powder without additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized L-Asparaginase in 18M-cm H2O at 1mg/ml.
Unit Definition
One unit of enzyme catalyzes hydrolyzation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.
Activity
One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37°C.
Specific Activity
225 U/mg.
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for ansA enzyme
L-Asparaginase is an enzyme that depletes L-Asparagine an important nutrient for cancer cells resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli, which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease
Description: L-Asparaginase produced from E. coli containing 303 amino acids and having a molecular mass of 31.73kD.
Description: L-Asparaginase produced from E. coli containing 303 amino acids and having a molecular mass of 31.73kD.
Product Categories/Family for ansA enzyme
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
L-asparaginase
NCBI Official Symbol
ansA
NCBI Protein Information
L-asparaginase
Protein Family
Similar Products
Product Notes
The ansA (Catalog #AAA654005) is an Enzyme produced from Escherichia Coli. and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "L-Asparaginase, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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