leptin N82K mutant Active Protein
Human leptin N82K mutant
Purity
Purity of recombinant human leptin N82K mutant is greater than 98.0% as determined by:(a) Gel filtration analysis of the recombinant protein.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
leptin N82K mutant; Human leptin N82K mutant; Human leptin N82K mutant LEP-15; leptin N82K mutant active protein
Host
Escherichia Coli
Purity/Purification
Purity of recombinant human leptin N82K mutant is greater than 98.0% as determined by:
(a) Gel filtration analysis of the recombinant protein.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
(a) Gel filtration analysis of the recombinant protein.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids of recombinant human leptin N82K mutant was determined and was found to be Ala-Val-Pro-Ile-Gln.
Solubility
It is recommended to reconstitute the lyophilized recombinant human leptin N82K mutant in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation in recombinant human leptin N82K mutant was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.87 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified recombinant human leptin (16K) N82K mutant consists of > 98% monomers as determined by gel-filtration chromatography.
Endotoxin
The endotoxin content of recombinant human leptin is less than 0.05 ng/ug (0.5 IEU/ug).
Formulation
The recombinant protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
recombinant human leptin N82K mutant is less than 0.1% active active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. This abolishment of activity results from drastically reduced affinity toward leptin receptor.
Preparation and Storage
Lyophilized recombinant human leptin N82K mutant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg human leptin per ml and up to 2 mg and filter sterilization recombinant human leptin N82K mutant can be stored at +4 degree C or even room temperature for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Related Product Information for leptin N82K mutant active protein
Recombinant protein - human leptin N82K mutant was produced by site specific mutagenesis. It consists of one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa., Human leptin mutant was purified by proprietary chromatographic techniques, see Niv-Spector et al. (2010) Mol Genet Metab. 100(2):193-7. It is devoid of biological activity and mey serve for control experiments.
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Product Notes
The leptin N82K mutant (Catalog #AAA400103) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids of recombinan t human leptin N82K mutant was determined and was found to be Ala-Val-Pr o-Ile-Gln. It is sometimes possible for the material contained within the vial of "leptin N82K mutant, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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