FGF-21 active protein

Recombinant Rat Fibroblast Growth Factor-21 (rRtFGF-21)

Cat. Num. AAA546405

• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Synonyms: FGF-21; Recombinant Rat Fibroblast Growth Factor-21 (rRtFGF-21); FGF-21 active protein

• Host: Escherichia coli.
• Purity/Purification: > 95% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.
• Sequence: AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

• Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 ug/ml of rMuKlotho-β and 10 ug/ml of heparin.
• Physical Appearance: White lyophilized powder.
• Endotoxin: Less than 1EU/ug of rRtFGF-21 as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Related Product Information for FGF-21 active protein

Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

NCBI and Uniprot Product Information

• NCBI GI #: 9506597
• NCBI GeneID: 26291
• NCBI Accession #: NP_061986.1
• NCBI GenBank Nucleotide #: NM_019113.3
• Molecular Weight: Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
• NCBI Official Full Name: fibroblast growth factor 21
• NCBI Official Synonym Full Names: fibroblast growth factor 21
• NCBI Official Symbol: FGF21
• NCBI Protein Information: fibroblast growth factor 21
• UniProt Protein Name: Fibroblast growth factor 21
• UniProt Gene Name: FGF21
• UniProt Synonym Gene Names: FGF-21
• UniProt Entry Name: FGF21_HUMAN

NCBI Description

Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cell; extracellular region; extracellular space

Molecular Function: fibroblast growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell-cell signaling; positive regulation of cell proliferation; positive regulation of glucose import; positive regulation of low-density lipoprotein receptor biosynthetic process; response to activity; response to nutrient levels; signal transduction; unfolded protein response