Rat ERO1-like protein alpha ELISA Kit | ERO1L elisa kit

Rat ERO1-like protein alpha (ERO1L) ELISA Kit

Cat. Num. AAA283714

• Gene Names: Ero1a; Ero1l
• Reactivity: Rat
• Synonyms: ERO1-like protein alpha; Rat ERO1-like protein alpha (ERO1L) ELISA Kit; ERO1-alpha; ERO1-like; ERO1L elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ERO1L. No significant cross-reactivity or interference between Rat ERO1L and analogues was observed.
• Sequence Length: 468

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ERO1L elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ERO1L in samples. An antibody specific for ERO1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyERO1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERO1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERO1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 19924061
• NCBI GeneID: 171562
• NCBI Accession #: NP_612537.1
• NCBI GenBank Nucleotide #: NM_138528.1
• UniProt Accession #: Q8R4A1; Q8VH29; Q8VH30
• Molecular Weight: 41,592 Da
• NCBI Official Full Name: ERO1-like protein alpha
• NCBI Official Synonym Full Names: endoplasmic reticulum oxidoreductase 1 alpha
• NCBI Official Symbol: Ero1a
• NCBI Official Synonym Symbols: Ero1l
• NCBI Protein Information: ERO1-like protein alpha
• UniProt Protein Name: ERO1-like protein alpha
• Protein Family: Ero1-like protein
• UniProt Gene Name: Ero1a
• UniProt Synonym Gene Names: ERO1-L; ERO1-L-alpha

NCBI Description

may function in ischemia-induced neuronal repair; may be involved in offsetting abnormalities in protein folding, distribution, or maturation [RGD, Feb 2006]

Uniprot Description

ERO1L: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT. Stimulated by hypoxia; suggesting that it is regulated via the HIF-pathway. Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum. Enzyme activity is tightly regulated to prevent the accumulation of reactive oxygen species in the endoplasmic reticulum. Reversibly down-regulated by the formation of disulfide bonds between the active site Cys-94 and Cys-131, and between Cys- 99 and Cys-104. Glutathione may be required to regulate its activity in the endoplasmic reticulum. Belongs to the EROs family.

Protein type: EC 1.8.4.-; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15p14

Cellular Component: dendrite; endoplasmic reticulum; endoplasmic reticulum membrane; intracellular; membrane

Molecular Function: oxidoreductase activity; oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor; protein disulfide isomerase activity; protein disulfide oxidoreductase activity

Biological Process: 4-hydroxyproline metabolic process; brown fat cell differentiation; cell redox homeostasis; cellular protein modification process; chaperone cofactor-dependent protein folding; endoplasmic reticulum unfolded protein response; extracellular matrix organization; organ senescence; protein folding; protein maturation via protein folding; release of sequestered calcium ion into cytosol