EPHB4 recombinant protein

Recombinant Human EPHB4 protein

Cat. Num. AAA1562389

• Gene Names: EPHB4; HTK; MYK1; TYRO11
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EPHB4; Recombinant Human EPHB4 protein; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11; EPHB4 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human EPHB4 (Leu16-Ala539) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for EPHB4 recombinant protein

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.

Product Categories/Family for EPHB4 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 32528301
• NCBI GeneID: 2050
• NCBI Accession #: NP_004435.3
• NCBI GenBank Nucleotide #: NM_004444.4
• UniProt Accession #: P54760; Q9BTA5; Q9BXP0
• Molecular Weight: 108,270 Da
• NCBI Official Full Name: ephrin type-B receptor 4
• NCBI Official Synonym Full Names: EPH receptor B4
• NCBI Official Symbol: EPHB4
• NCBI Official Synonym Symbols: HTK; MYK1; TYRO11
• NCBI Protein Information: ephrin type-B receptor 4; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB4
• UniProt Synonym Gene Names: HTK; MYK1; TYRO11
• UniProt Entry Name: EPHB4_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; cytosol

Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: heart morphogenesis; axon guidance; peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; protein amino acid autophosphorylation; ephrin receptor signaling pathway; angiogenesis; cell adhesion