EphA7/EHK3 recombinant protein

Recombinant Human EphA7/EHK3 Protein (C-His)

Cat. Num. AAA2557298

• Gene Names: EPHA7; EHK3; EK11; EHK-3; HEK11
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: EphA7/EHK3; Recombinant Human EphA7/EHK3 Protein (C-His); Ephrin Type-A Receptor 7; EPH Homology Kinase 3; EHK-3; EPH-Like Kinase 11; EK11; hEK11; EPHA7; EHK3; HEK11; EphA7/EHK3 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: Gln28-Ile556
• Sequence Length: 998

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for EphA7/EHK3 recombinant protein

Protein Construction: Recombinant Human EphA7 is produced by our mammalian expression system and the target gene encoding Gln28-Ile556 is expressed with a 6His tag at the C-terminus.

Background: Ephrin Type-A Receptor 7 (EPHA7) is a single-pass type I membrane protein which belongs to the Eph family of transmembrane receptor tyrosine kinases. It contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA7 is a receptor for members of the ephrin-A family. Eph receptors are largely expressed throughout the ectoderm, mesoderm, and endoderm of vertebrate embryos. EPHA7 functions as a repulsive guidance molecule during the targeting of retinal axons to the superior colliculus and of neocortical axons to the thalamus. EPHA7 is expressed at a substantial level in most human lung cancers. The high expression of EPHA7 protein may participate in the malignancy transformation, invasion progression and metastasis of primary hepatocellular carcinoma. EPHA7 may involve in smoking related lung carcinogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4758282
• NCBI GeneID: 2045
• NCBI Accession #: NP_004431.1
• NCBI GenBank Nucleotide #: NP_004431.1
• UniProt Accession #: Q15375
• Molecular Weight: Molecular Mass: 60.19kDa; Actual Protein Molecular Mass: 72kDa
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 1
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; EK11; EHK-3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7
• UniProt Protein Name: Ephrin type-A receptor 7
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11
• UniProt Entry Name: EPHA7_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; dendrite; plasma membrane; neuromuscular junction

Molecular Function: protein binding; ephrin receptor binding; chemorepellent activity; protein-tyrosine kinase activity; GPI-linked ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; branching morphogenesis of a nerve; regulation of protein amino acid autophosphorylation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; negative chemotaxis; brain development; regulation of caspase activity; phosphorylation; regulation of peptidyl-tyrosine phosphorylation; retinal ganglion cell axon guidance