Human EPH receptor A4 ELISA Kit | EPHA4 elisa kit

Human Ephrin type-A receptor 4, EPHA4 ELISA Kit

Cat. Num. AAA914925

• Gene Names: EPHA4; SEK; HEK8; TYRO1
• Reactivity: Human
• Synonyms: EPH receptor A4; Human Ephrin type-A receptor 4; EPHA4 ELISA Kit; Human Ephrin type-A receptor 4 (EPHA4) ELISA kit; HEK8; SEK; TYRO1; OTTHUMP00000164185; TYRO1 protein tyrosine kinase; ephrin receptor EphA4; ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; tyrosine-protein kinase receptor SEK; EPHA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EPHA4. No significant cross-reactivity or interference between human EPHA4 and analogues was observed.
• Sequence Length: 986

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 25 pg/ml-1600 pg/ml
• Sensitivity: < 6.25 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPHA4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4758280
• NCBI GeneID: 2043
• NCBI Accession #: NP_004429.1
• NCBI GenBank Nucleotide #: NM_004438.3
• UniProt Accession #: P54764; Q2M380; A8K2P1; B2R601
• Molecular Weight: 109,860 Da
• NCBI Official Full Name: ephrin type-A receptor 4
• NCBI Official Synonym Full Names: EPH receptor A4
• NCBI Official Symbol: EPHA4
• NCBI Official Synonym Symbols: SEK; HEK8; TYRO1
• NCBI Protein Information: ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8
• UniProt Protein Name: Ephrin type-A receptor 4
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA4
• UniProt Synonym Gene Names: HEK8; SEK; TYRO1; EK8; hEK8
• UniProt Entry Name: EPHA4_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; TK group; Eph family

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; early endosome membrane; axon; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium

Molecular Function: identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; ephrin receptor signaling pathway; regulation of astrocyte differentiation; motor axon guidance; positive regulation of dendrite morphogenesis; cell adhesion; corticospinal tract morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior