Human Prostaglandin E Receptor 2 ELISA Kit | EP2 elisa kit

Human Prostaglandin E Receptor 2 ELISA Kit

Cat. Num. AAA765106

• Gene Names: PTGER2; EP2
• Reactivity: Human
• Synonyms: Prostaglandin E Receptor 2; Human Prostaglandin E Receptor 2 ELISA Kit; EP2/PGE receptor EP2 subtype/PGE2 receptor EP2 subtype/prostaglandin E receptor 2 (subtype EP2); 53kDa/Prostanoid EP2 receptor; EP2 elisa kit

• Reactivity: Human
• Sequence Length: 358

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EP2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EP2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EP2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EP2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EP2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 30526196
• NCBI GeneID: 5732
• Molecular Weight: 39,761 Da
• NCBI Official Full Name: prostaglandin E receptor 2 subtype EP2
• NCBI Official Synonym Full Names: prostaglandin E receptor 2
• NCBI Official Symbol: PTGER2
• NCBI Official Synonym Symbols: EP2
• NCBI Protein Information: prostaglandin E2 receptor EP2 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP2 subtype
• UniProt Gene Name: PTGER2
• UniProt Synonym Gene Names: PGE receptor EP2 subtype; PGE2 receptor EP2 subtype
• UniProt Entry Name: PE2R2_HUMAN

NCBI Description

This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma. [provided by RefSeq, Oct 2009]

Uniprot Description

PTGER2: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 14q22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; regulation of cell proliferation; response to lipopolysaccharide; response to progesterone stimulus

Disease: Asthma, Nasal Polyps, And Aspirin Intolerance