Testing Data
Cloning Enzyme
Ligation-Free Cloning Kit
Applications
multiple fragment cloning and assembly.cloning of any insert into any location of a chosen vector.
complete elimination of the dependence on availability of restriction sites, phosphatase treatment and ligation.
inserts free from any redundant o
Synonyms
Cloning; Ligation-Free Cloning Kit; Cloning enzyme
Applicable Applications for Cloning enzyme
multiple fragment cloning and assembly.
cloning of any insert into any location of a chosen vector.
complete elimination of the dependence on availability of restriction sites, phosphatase treatment and ligation.
inserts free from any redundant o
cloning of any insert into any location of a chosen vector.
complete elimination of the dependence on availability of restriction sites, phosphatase treatment and ligation.
inserts free from any redundant o
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Cloning enzyme
The Ligation-Free Cloning kit is a versatile system to meet the demands of routine cloning projects. The progressive design has eliminated the requirement for compatible ends and unique restriction sites within the vector and inserts to be cloned. At the foundation of Ligation-Free Cloning technology is proprietary Ligation-Free Enzyme Mix which reliably facilitates the assembly of up to 3 DNA inserts (PCR-generated sequences) and a linearized vector by recognizing a 15 bp overlap region at both ends. This 15 bp overlap can be engineered by designing primers for amplification of your desired sequences to create inserts free from any redundant or unwanted base pairs. To eliminate the possibility of mutations associated with PCR amplification, scientists have developed a DNA Polymerase with the highest proof-reading capability thus making this system the most reliable on the market.
Similar Products
Product Notes
The Cloning (Catalog #AAA4156413) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cloning can be used in a range of immunoassay formats including, but not limited to, multiple fragment cloning and assembly. cloning of any insert into any location of a chosen vector. complete elimination of the dependence on availability of restriction sites, phosphatase treatment and ligation. inserts free from any redundant o. Researchers should empirically determine the suitability of the Cloning for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cloning, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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