Autotaxin / ENPP2 / NPP2 Recombinant Protein | ENPP2 / NPP2 recombinant protein

Recombinant Human Autotaxin / ENPP2 / NPP2 Protein (His tag)

Cat. Num. AAA2545817

• Gene Names: ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: Autotaxin / ENPP2 / NPP2; Recombinant Human Autotaxin / ENPP2 / NPP2 Protein (His tag); ATX; ATX-X; AUTOTAXIN; LysoPLD; NPP2; PD-IALPHA; PDNP2; ENPP2 / NPP2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 863

• Application Notes: The recombinant human ENPP2 consists of 834 amino acids and has a calculated molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhENPP2 is approximately 120-130 kDa due to glycosylation.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for ENPP2 / NPP2 recombinant protein

Background: ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2), also referred as Autotaxin, is a secreted enzyme encoded by the ENPP2 gene. This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. ATX has been found to catalyze the formation of cyclic phosphatidic acid (cPA), which have antitumor role by antimitogenic regulation of cell cycle, inhibition of cancer invasion and metastasis. LPA receptors and ATX are upregulated in numerous cancer cell type s and show expression patterns that correlate with tumor cell invasiveness. Thus, Autotaxin has recently emerged as an attractive target for the development of anti-cancer chemotherapeutics. In addition, Serum ATX activity was found to be enhanced in relation to hepatic fibrosis in chronic liver disease due to hepatitis virus C infection.

Description: A DNA sequence encoding the mature form of human ENPP2 (AAH34961.1) (Asp 49-Ile 863) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 22137786
• NCBI GeneID: 5168
• Molecular Weight: 101,968 Da
• NCBI Official Full Name: Ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Synonym Full Names: ectonucleotide pyrophosphatase/phosphodiesterase 2
• NCBI Official Symbol: ENPP2
• NCBI Official Synonym Symbols: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
• NCBI Protein Information: ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• UniProt Protein Name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• UniProt Gene Name: ENPP2
• UniProt Synonym Gene Names: ATX; PDNP2; E-NPP 2; LysoPLD

NCBI Description

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2008]

Uniprot Description

ENPP2: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.39; Lipid Metabolism - ether lipid; Motility/polarity/chemotaxis; Phosphodiesterase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.12

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: calcium ion binding; hydrolase activity; lysophospholipase activity; transcription factor binding; zinc ion binding

Biological Process: cell motility; G-protein coupled receptor protein signaling pathway; phosphate metabolic process; phospholipid catabolic process; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell migration