ENOA Blocking Peptide | ENO1 blocking peptide

ENOA Antibody (C-term) Blocking Peptide

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
• Synonyms: ENOA; ENOA Antibody (C-term) Blocking Peptide; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1; ENO1L1; MBPB1; MPB1; ENO1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human ENOA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 434

• Cellular Location: Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
• Tissue Location: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ENO1 blocking peptide

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

NCBI and Uniprot Product Information

• NCBI GI #: 119339
• NCBI GeneID: 2023
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• Molecular Weight: 36,928 Da
• NCBI Official Full Name: Alpha-enolase
• NCBI Official Synonym Full Names: enolase 1
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1; HEL-S-17
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1
• UniProt Protein Name: Alpha-enolase
• Protein Family: Enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: EC 4.2.1.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transcription, coactivator/corepressor; Lyase

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: cytoplasm; cytosol; extracellular space; membrane; nucleus

Molecular Function: GTPase binding; phosphopyruvate hydratase activity; protein binding; transcription corepressor activity; transcription factor activity

Biological Process: gluconeogenesis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to virus