Typical Testing Data/Standard Curve (for reference only)
Rabbit Enkephalin ELISA Kit | ENK elisa kit
Rabbit Enkephalin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ENK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ENK antibody and an ENK-HRP conjugate. The assay sample and buffer are incubated together with ENK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ENK concentration since ENK from samples and ENK-HRP conjugate compete for the anti-ENK antibody binding site. Since the number of sites is limited, as more sites are occupied by ENK from the sample, fewer sites are left to bind ENK-HRP conjugate. A standard curve is p lotted relating the intensity of the color (O.D.) to the concentration of standards. The ENK concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
neuropeptide hormone; plays a role in the responses to stress and pain perception [RGD, Feb 2006]
Uniprot Description
Function: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(239-260) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Ref.8
Subcellular location: Secreted.
Tissue specificity: Expressed in brain, heart and testis.
Post-translational modification: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Sequence similarities: Belongs to the opioid neuropeptide precursor family.
Mass spectrometry: Molecular mass is 2203.971 Da from positions 114 - 133. Determined by ESI. Ref.8Molecular mass is 4592.8 Da from positions 143 - 185. Determined by ESI. Ref.8Molecular mass is 2504.027 Da from positions 239 - 260. Determined by ESI. Ref.8
Research Articles on ENK
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Product Notes
The Rabbit ENK penk (Catalog #AAA727404) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727404 ELISA Kit recognizes Rabbit ENK. It is sometimes possible for the material contained within the vial of "Enkephalin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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