Typical Testing Data/Standard Curve (for reference only)
Human elastin ELISA Kit | ELN elisa kit
Human Elastin, ELN ELISA Kit
Gene Names
ELN; WS; WBS; SVAS
Reactivity
Human
Synonyms
elastin; Human Elastin; ELN ELISA Kit; FLJ38671; FLJ43523; SVAS; WBS; WS; OTTHUMP00000211864; tropoelastin; ELN elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human ELN. No significant cross-reactivity or interference between human ELN and analogues was observed.
Sequence Length
724
Samples
Serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
< 0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ELN elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ELN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ELN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELN bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,469 Da
NCBI Official Full Name
elastin isoform a
NCBI Official Synonym Full Names
elastin
NCBI Official Symbol
ELN
NCBI Official Synonym Symbols
WS; WBS; SVAS
NCBI Protein Information
elastin; tropoelastin
UniProt Protein Name
Elastin
Protein Family
UniProt Gene Name
ELN
UniProt Entry Name
ELN_HUMAN
NCBI Description
This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle
Research Articles on ELN
1. Tropoelastin modulates TGF-beta1-induced expression of VEGF and CTGF in airway smooth muscle cells.
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Product Notes
The Human ELN eln (Catalog #AAA704171) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704171 ELISA Kit recognizes Human ELN. It is sometimes possible for the material contained within the vial of "elastin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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