SerpinA3 elisa pair set

SerpinA3 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118231

• Synonyms: SerpinA3; SerpinA3 Matched ELISA Antibody Pair Set; Human; Human SerpinA3 ELISA Pair Set; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 3; AACT Matched ELISA Antibody Pair Set; ACT Matched ELISA Antibody Pair Set; GIG24 Matched ELISA Antibody Pair Set; GIG25 Matched ELISA Antibody Pair Set; MGC88254 Matched ELISA Antibody Pair Set; SERPINA3 Matched ELISA Antibody Pair Set; SerpinA3 elisa pair set

• Specificity: Quantitative determination of Human SerpinA3

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 47-3000pg/mL
• Sensitivity: 47pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human SerpinA3 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human SerpinA3 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 170 ng of recombinant Human SerpinA3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for SerpinA3 elisa pair set

SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Besides, ACT gene polymorphism has been implicated with Alzheimer's disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.

This SerpinA3 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human SerpinA3. It contains Human SerpinA3 capture antibody, Human SerpinA3 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human SerpinA3 protein.

References

Nilsson, L.N. et al., 2001, J. Neurosci. 21: 1444-1451. Ikari, Y. et al., 2001, J. Biol. Chem. 276: 11798-11803. Vila, N. et al., 2000, Stroke. 31: 2103-2105 Eriksson, S. et al., 1995, Proc. Natl. Acad. Sci. USA. 92: 2313-2317. Skeel, A. et al., 2001, J. Biol. Chem. 276: 21932-21937.