Testing Data
IL13Ra1 elisa pair set
IL13RA1 Matched ELISA Antibody Pair Set, Human
Synonyms
IL13RA1; IL13RA1 Matched ELISA Antibody Pair Set; Human; Human IL13RA1/CD213a1 ELISA Pair Set; interleukin 13 receptor; alpha 1; CD213A1 Matched ELISA Antibody Pair Set; IL-13Ra Matched ELISA Antibody Pair Set; NR4 Matched ELISA Antibody Pair Set; IL13Ra1 elisa pair set
Specificity
Quantitative determination of Human IL13RA1
Assay Type
Solid Phase Sandwich ELISA
Detection Range
31.25-2000pg/mL
Sensitivity
31.25pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-IL13RA1 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL of mouse anti-IL13RA1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 80 ng of recombinant IL13RA1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL of mouse anti-IL13RA1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 80 ng of recombinant IL13RA1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for IL13Ra1 elisa pair set
Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2 and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13). This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
This IL13RA1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL13RA1. It contains Human IL13RA1 capture antibody, Human IL13RA1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL13RA1 protein.
This IL13RA1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL13RA1. It contains Human IL13RA1 capture antibody, Human IL13RA1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL13RA1 protein.
Product Categories/Family for IL13Ra1 elisa pair set
References
Kawakami M, et al. (2002) Mutation and functional analysis of IL-13 receptors in human malignant glioma cells. Oncol Res. 12 (11-12): 459-67.Umeshita-Suyama R, et al. (2000) Characterization of IL-4 and IL-13 signals dependent on the human IL-13 receptor alpha chain 1: redundancy of requirement of tyrosine residue for STAT3 activation. Int Immunol. 12 (11): 1499-509.He JQ, et al. (2003) Polymorphisms in the IL13, IL13RA1, and IL4RA genes and rate of decline in lung function in smokers. Am J Respir. Cell Mol Biol. 28 (3): 379-85.
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Product Notes
The IL13Ra1 (Catalog #AAA8118329) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL13RA1, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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