PSA elisa kit
PSA ELISA Kit
Applications
ELISA
Synonyms
PSA; PSA ELISA Kit; Prostate Specific Antigen ELISA Kit; Total PSA ELISA Kit; PSA elisa kit
Applicable Applications for PSA elisa kit
ELISA (EIA)
Application Notes
The PSA ELISA test is a solid phase two-site immunoassay. An anti-PSA monoclonal antibody is coated on the surface of the microtiter wells and a mouse anti-PSA antibody labeled with horseradish peroxidase is used as the tracer. The PSA molecules present in the standard solution or sera are 'sandwiched' between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound proteins and antibody-enzyme tracers are removed by washing. The horseradish peroxidase activity bound in the wells is then assayed by a colorimetric reaction. The intensity of the color formed is proportional to the concentration of PSA present in the sample.
Biological Significance
PSA is a 32 kDa single chain glycoprotein serine protease with a chymotrypsin like specificity produced by the secretory epithelium of the prostate gland. Only low levels of PSA are normally present in the blood stream, and increasing serum concentrations indicate prostatic pathology, including benign prostatic hyperplasia and cancer of the prostate.
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
Preparation and Storage
Store at 4 degree C.
Related Product Information for PSA elisa kit
ELISA kit for detection of PSA in the research laboratory
Product Categories/Family for PSA elisa kit
Similar Products
Product Notes
The PSA (Catalog #AAA539744) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PSA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The PSA ELISA test is a solid phase two-site immunoassay. An anti-PSA monoclonal antibody is coated on the surface of the microtiter wells and a mouse anti-PSA antibody labeled with horseradish peroxidase is used as the tracer. The PSA molecules present in the standard solution or sera are 'sandwiched' between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound proteins and antibody-enzyme tracers are removed by washing. The horseradish peroxidase activity bound in the wells is then assayed by a colorimetric reaction. The intensity of the color formed is proportional to the concentration of PSA present in the sample. Researchers should empirically determine the suitability of the PSA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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