HEV-Ag elisa kit
HEV-Ag ELISA Kit
Synonyms
HEV-Ag; HEV-Ag ELISA Kit; HEV-Ag elisa kit
Samples
Serum, plasma
Assay Type
Qualitative Sandwich
Preparation and Storage
+4C
Related Product Information for HEV-Ag elisa kit
Principle of the Assay: This HEV Ag ELISA kit uses polystyrene microwell strips pre-coated with rabbit anti-HEV antibodies directed against the structural proteins ORF-2 of the native virus. Patient's serum, plasma or PBS dissolved stool sample is added into the microwells. In case of presence of HEV Ag in the sample, the pre-coated antibodies will be bound to the viral antigen and during the first incubation step, the specific immunocomplex formed is captured on the solid phase. After washing to remove unbound sample, second, monoclonal anti-HEV antibody conjugated to Horseradish Peroxidase (HRP) is added into the wells. During the second incubation step, this antibody will bind to the HEV antigens if they have been captured by anti-HEV antibodies during the first incubation step. The unbound HRP conjugate is removed during washing and Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antibody-antigen-antibody (HRP) "sandwich" complex, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for HEV antigens remain colorless.!!Background/Introduction: This HEV Ag kit is an enzyme-linked immunosorbent assay for the qualitative detection of hepatitis E viral antigen (HEV Ag) in human serum, plasma, or stool samples. For Research Use Only
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Product Notes
The HEV-Ag (Catalog #AAA412749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HEV-Ag, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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