Coxiella burnetii Phase I IgG ELISA Kit | IgG elisa kit
Coxiella burnetii Phase I IgG ELISA Kit
Synonyms
Coxiella burnetii Phase I IgG; Coxiella burnetii Phase I IgG ELISA Kit; IgG elisa kit
Specificity
The Coxiella burnetii ELISA test kits was verified in an internal study. The sensitivity and specificity in all cases exceeded 94%.
Samples
Various Kinds Of Samples.
Preparation and Storage
2 - 8 degree C
Related Product Information for IgG elisa kit
Intended Uses: The Coxiella burnetii tests are qualitative ELISAs for the demonstration of human antibodies directed against Coxiella burnetii in phase 1 or phase 2. These immunoassays are recommended for the sensitive detection of such antibodies in various kinds of samples.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: The Rickettsia Coxiella burnetii, a worldwide distributed pathogen, is responsible for the disease known as Q fever. The small gram-negative, obligate intracellular bacterium reproduces in the digestive system of ticks (Dermacentor marginatus) and placental trophoblasts. As a result tick faeces and afterbirths of infected mammals (particularly sheep) are highly infectious. ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in various kinds of samples. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: The Rickettsia Coxiella burnetii, a worldwide distributed pathogen, is responsible for the disease known as Q fever. The small gram-negative, obligate intracellular bacterium reproduces in the digestive system of ticks (Dermacentor marginatus) and placental trophoblasts. As a result tick faeces and afterbirths of infected mammals (particularly sheep) are highly infectious. ELISA test-systems are especially suited for the differential analysis of immunoglobulin classes directed against the virus.
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Product Notes
The IgG (Catalog #AAA495213) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Coxiella burnetii Phase I IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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