Neutrophil elastase Recombinant Protein | ELNE recombinant protein
Recombinant Human Neutrophil elastase
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
NCBI and Uniprot Product Information
NCBI Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
Uniprot Description
ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.
Protein type: Cell cycle regulation; Cell surface; EC 3.4.21.37; Motility/polarity/chemotaxis; Protease
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; secretory granule; transcriptional repressor complex
Molecular Function: cytokine binding; endopeptidase activity; heparin binding; peptidase activity; protease binding; protein binding; serine-type endopeptidase activity
Biological Process: acute inflammatory response to antigenic stimulus; antimicrobial humoral response; biosynthetic process of antibacterial peptides active against Gram-negative bacteria; defense response to bacterium; extracellular matrix disassembly; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; neutrophil degranulation; phagocytosis; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to UV
Disease: Cyclic Neutropenia; Neutropenia, Severe Congenital, 1, Autosomal Dominant
Research Articles on ELNE
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Product Notes
The ELNE elane (Catalog #AAA9420208) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length, 30-267aa. The Recombinant Human Neutrophil elastase reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: IVGGRRARPH AWPFMVSLQL RGGHFCGATL IAPNFVMSAA HCVANVNVRA VRVVLGAHNL SRREPTRQVF AVQRIFENGY DPVNLLNDIV ILQLNGSATI NANVQVAQLP AQGRRLGNGV QCLAMGWGLL GRNRGIASVL QELNVTVVTS LCRRSNVCTL VRGRQAGVCF GDSGSPLVCN GLIHGIASFV RGGCASGLYP DAFAPVAQFV NWIDSIIQRS EDNPCPHPRD PDPASRTH. It is sometimes possible for the material contained within the vial of "Neutrophil elastase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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