Testing Data
EK recombinant protein
EK (enterokinase), Active
Applications
In vitro Proteolysis
Synonyms
EK; EK (enterokinase); Active; EK recombinant protein
Host
Yeast cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 8, 200mM NaCl, 30% glycerol.
Sequence Positions
800-end
Applicable Applications for EK recombinant protein
In vitro Proteolysis
Species
Swine
Tag
HIS tag
Enzyme Commission Number
EC 3.4.21.9
Preparation and Storage
Store product at -20 degree C for up to 1 year. Aliquot enzymes to avoid freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for EK recombinant protein
Description: Recombinant swine EK (800-end) was expressed in yeast cells using an N-terminal His tag.
Scientific Background: Enterokinase (EK) is a member of the trypsin family of serine proteases, which cleaves proteins following the Lys at the Asp-Asp-Asp-Asp-Lys recognition sequence. In the stomach, EK converts trypsinogen into trypsin. EK has high specificity and high hydrolytic efficiency, making it a widely used biochemical tool for cleaving recombinant fusion proteins. EK can tolerate a variety of detergents and some denaturing agents. EK's activity is reduced by > 250 mM NaCl, > 2 M urea, > 20 mM, beta-ME, > 0.1% SDS and > 250 mM imidazole.
Scientific Background: Enterokinase (EK) is a member of the trypsin family of serine proteases, which cleaves proteins following the Lys at the Asp-Asp-Asp-Asp-Lys recognition sequence. In the stomach, EK converts trypsinogen into trypsin. EK has high specificity and high hydrolytic efficiency, making it a widely used biochemical tool for cleaving recombinant fusion proteins. EK can tolerate a variety of detergents and some denaturing agents. EK's activity is reduced by > 250 mM NaCl, > 2 M urea, > 20 mM, beta-ME, > 0.1% SDS and > 250 mM imidazole.
Product Categories/Family for EK recombinant protein
Similar Products
Product Notes
The EK (Catalog #AAA515500) is a Recombinant Protein produced from Yeast cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 800-end. AAA Biotech's EK can be used in a range of immunoassay formats including, but not limited to, In vitro Proteolysis. Researchers should empirically determine the suitability of the EK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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