EIF5A recombinant protein

eIF5A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121808

• Gene Names: EIF5A; EIF-5A; EIF5A1; eIF5AI
• Purity: >85% as determined by SDS-PAGE
• Synonyms: eIF5A; eIF5A Protein; Human; Recombinant (His Tag); Human EIF5A Protein (His Tag); EIF-5A Protein; EIF5A1 Protein; eIF5AI Protein; eukaryotic translation initiation factor 5A; EIF5A recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys154

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human EIF5A (NP_001961.1)(Met1-Lys154) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EIF5A recombinant protein

Background: EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2-GTP-tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit.

References

Jenkins ZA. et al., 2001, Genomics. 71 (1): 101-9. Guan XY. et al., 2001, Cancer Res. 61 (9): 3806-9. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Clement PM. et al., 2003, Eur J Biochem. 270 (21): 4254-63.

NCBI and Uniprot Product Information

• NCBI GI #: 4503545
• NCBI GeneID: 1984
• NCBI Accession #: NP_001961.1
• NCBI GenBank Nucleotide #: NM_001970.4
• UniProt Accession #: P63241; P10159; Q16182; Q7L7L3; Q7Z4L1; Q9D0G2; A8K9A0; D3DTP2; AAB29229
• Molecular Weight: 20,170 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5A-1 isoform B
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5A
• NCBI Official Symbol: EIF5A
• NCBI Official Synonym Symbols: EIF-5A; EIF5A1; eIF5AI
• NCBI Protein Information: eukaryotic translation initiation factor 5A-1; eIF-4D; eIF-5A1; eIF-5A-1; rev-binding factor; eukaryotic initiation factor 5A
• UniProt Protein Name: Eukaryotic translation initiation factor 5A-1
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF5A
• UniProt Synonym Gene Names: eIF-5A-1; eIF-5A1; eIF-5A
• UniProt Entry Name: IF5A1_HUMAN

Uniprot Description

EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 17p13-p12

Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; cytosol; nucleus; annulate lamellae

Molecular Function: protein binding; RNA binding; ribosome binding; protein N-terminus binding; translation elongation factor activity; U6 snRNA binding

Biological Process: mRNA export from nucleus; translational frameshifting; cellular protein metabolic process; apoptosis; positive regulation of cell proliferation; nucleocytoplasmic transport; positive regulation of translational elongation; hypusine biosynthetic process from peptidyl-lysine; protein export from nucleus; post-translational protein modification; positive regulation of translational termination