Rat Eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit | Eif2ak3 elisa kit

Rat Eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit

Cat. Num. AAA2881340

• Gene Names: Eif2ak3; PEK
• Reactivity: Rat
• Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 3; Rat Eukaryotic translation initiation factor 2-alpha kinase 3 ELISA Kit; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; Eif2ak3; Pek; Perk; 2.7.11.1; Eif2ak3 elisa kit

• Reactivity: Rat
• Sequence Length: 1108

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Related Product Information for Eif2ak3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13928816
• NCBI GeneID: 29702
• NCBI Accession #: NP_113787.1
• NCBI GenBank Nucleotide #: NM_031599.2
• UniProt Accession #: Q9Z1Z1
• Molecular Weight: 124,770 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 alpha kinase 3
• NCBI Official Symbol: Eif2ak3
• NCBI Official Synonym Symbols: PEK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• Protein Family: Eukaryotic translation initiation factor 2-alpha kinase
• UniProt Gene Name: Eif2ak3
• UniProt Synonym Gene Names: Pek; Perk
• UniProt Entry Name: E2AK3_RAT

NCBI Description

kinase that phosphorylates eukaryotic initiation factor 2alpha; has an important role in translational control [RGD, Feb 2006]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, Other; Membrane protein, integral; Other group; PEK family; PEK subfamily

Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; perinuclear region of cytoplasm

Molecular Function: ATP binding; enzyme binding; eukaryotic translation initiation factor 2alpha kinase activity; Hsp90 protein binding; identical protein binding; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: angiogenesis; bone mineralization; calcium-mediated signaling; caspase activation; cellular response to amino acid starvation; cellular response to glucose starvation; chondrocyte development; endocrine pancreas development; endoplasmic reticulum organization and biogenesis; ER overload response; fat cell differentiation; insulin-like growth factor receptor signaling pathway; lactation; negative regulation of apoptosis; negative regulation of myelination; negative regulation of translation; negative regulation of translation in response to stress; ossification; pancreas development; peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of signal transduction; positive regulation of transcription from RNA polymerase I promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein homooligomerization; regulation of fatty acid metabolic process; skeletal development; SREBP-mediated signaling pathway; translation; unfolded protein response