Bovine 116 kDa U5 small nuclear ribonucleoprotein component ELISA Kit | EFTUD2 elisa kit
Bovine 116 kDa U5 small nuclear ribonucleoprotein component (EFTUD2) ELISA Kit
Reactivity
Bovine
Synonyms
116 kDa U5 small nuclear ribonucleoprotein component; Bovine 116 kDa U5 small nuclear ribonucleoprotein component (EFTUD2) ELISA Kit; EFTUD2 elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine EFTUD2. No significant cross-reactivity or interference between Bovine EFTUD2 and analogues was observed.
Sequence Length
962
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EFTUD2 elisa kit
EFTUD2 is structurally closely related to the eukaryotic translation elongation factor EF2, sharing 27 to 50% sequence identity depending on the region examined. this protein bound GTP specifically. Immunofluorescence microscopy revealed that U5-116kD is located in the nucleus of HeLa cells and colocalizes with snRNP-containing nuclear speckles.
Antibodies against U5-116kD inhibited pre-mRNA splicing in a HeLa nuclear extract. These authors also identified homologs in S. cerevisiae, C. elegans, and mouse, designating them Snu114p, Caeel-116H, and U5-116kD, respectively. Human U5-116kD shares 32%, 74%, and 99% identity with Snu114p, Caeel-116H, and mouse U5-116kD, respectively
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EFTUD2 in samples. An antibody specific for EFTUD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFTUD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFTUD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFTUD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Antibodies against U5-116kD inhibited pre-mRNA splicing in a HeLa nuclear extract. These authors also identified homologs in S. cerevisiae, C. elegans, and mouse, designating them Snu114p, Caeel-116H, and U5-116kD, respectively. Human U5-116kD shares 32%, 74%, and 99% identity with Snu114p, Caeel-116H, and mouse U5-116kD, respectively
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EFTUD2 in samples. An antibody specific for EFTUD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFTUD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFTUD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFTUD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109,386 Da
NCBI Official Full Name
116 kDa U5 small nuclear ribonucleoprotein component
NCBI Official Synonym Full Names
elongation factor Tu GTP binding domain containing 2
NCBI Official Symbol
EFTUD2
NCBI Protein Information
116 kDa U5 small nuclear ribonucleoprotein component
UniProt Protein Name
116 kDa U5 small nuclear ribonucleoprotein component
Protein Family
UniProt Gene Name
EFTUD2
UniProt Synonym Gene Names
SNRP116; U5-116 kDa
Uniprot Description
Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex required for pre-mRNA splicing. Binds GTP ().
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Product Notes
The Bovine EFTUD2 eftud2 (Catalog #AAA281191) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281191 ELISA Kit recognizes Bovine EFTUD2. It is sometimes possible for the material contained within the vial of "116 kDa U5 small nuclear ribonucleoprotein component, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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