EFNA5 recombinant protein

Recombinant Human EFNA5 Protein

Cat. Num. AAA8248957

• Gene Names: EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: EFNA5; Recombinant Human EFNA5 Protein; EPLG7; LERK7; Ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7; EFNA5 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: QDPGSKAVAD RYAVYWNSSN PRFQRGDYHI DVCINDYLDV FCPHYEDSVP EDKTERYVLY MVNFDGYSAC DHTSKGFKRW ECNRPHSPNG PLKFSEKFQL FTPFSLGFEF RPGREYFYIS SAIPDNGRRS CLKLKVFVRP TNSCMKTIGV HDRVFDVNDK VENSLEPADD TVHESAEPSR GENVDDIEGR MDEPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK
• Sequence Length: 228

• Applicable Applications for EFNA5 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for EFNA5 recombinant protein

Recombinant Human EFNA5 Protein is produced by our mammalian expression system and the target gene encoding Gln21-Asn203 is expressed with a Fc tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4503487
• NCBI GeneID: 1946
• NCBI Accession #: NP_001953.1
• NCBI GenBank Nucleotide #: NM_001962.2
• UniProt Accession #: P52803
• Molecular Weight: 26,297 Da
• NCBI Official Full Name: ephrin-A5
• NCBI Official Synonym Full Names: ephrin A5
• NCBI Official Symbol: EFNA5
• NCBI Official Synonym Symbols: AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• NCBI Protein Information: ephrin-A5
• UniProt Protein Name: Ephrin-A5
• Protein Family: Ephrin
• UniProt Gene Name: EFNA5
• UniProt Synonym Gene Names: EPLG7; LERK7; LERK-7
• UniProt Entry Name: EFNA5_HUMAN

NCBI Description

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA5: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. Belongs to the ephrin family.

Protein type: Cell development/differentiation; Ligand, receptor tyrosine kinase; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: anchored to external side of plasma membrane; extracellular region; plasma membrane

Molecular Function: ephrin receptor binding

Biological Process: apoptosis; axon guidance; ephrin receptor signaling pathway; nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell morphogenesis; regulation of cell-cell adhesion; regulation of focal adhesion formation; regulation of GTPase activity