EEF1B2 recombinant protein

EEF1B2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121744

• Gene Names: EEF1B2; EF1B; EEF1B; EEF1B1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: EEF1B2; EEF1B2 Protein; Human; Recombinant (His Tag); Human EF1B/EEF1B2 Protein (His Tag); EEF1B Protein; EEF1B1 Protein; EF1B Protein; eukaryotic translation elongation factor 1 beta 2; EEF1B2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, 1mM DTT, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile225

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human EEF1B2 (P24534)(Met1-Ile225) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EEF1B2 recombinant protein

Background: EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.

References

Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62. Rual. et al., 2005, Nature. 437 (7062): 1173-8. Stelzl. et al., 2005, Cell. 122 (6): 957-68. Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.

NCBI and Uniprot Product Information

• NCBI GI #: 83376130
• NCBI GeneID: 1933
• NCBI Accession #: NP_001032752.1
• NCBI GenBank Nucleotide #: NM_001037663.1
• UniProt Accession #: P24534; Q6IBH9; A8K795
• Molecular Weight: 24,764 Da
• NCBI Official Full Name: elongation factor 1-beta
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 beta 2
• NCBI Official Symbol: EEF1B2
• NCBI Official Synonym Symbols: EF1B; EEF1B; EEF1B1
• NCBI Protein Information: elongation factor 1-beta; EF-1-beta; eukaryotic translation elongation factor 1 beta 1
• UniProt Protein Name: Elongation factor 1-beta
• UniProt Gene Name: EEF1B2
• UniProt Synonym Gene Names: EEF1B; EF1B; EF-1-beta
• UniProt Entry Name: EF1B_HUMAN

NCBI Description

This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR. [provided by RefSeq, Jul 2008]

Uniprot Description

eEF1B: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; translation; gene expression