Human Extracellular matrix protein 1 ELISA Kit | ECM1 elisa kit

Human Extracellular matrix protein 1 ELISA Kit

Cat. Num. AAA763045

• Reactivity: Human
• Synonyms: Extracellular matrix protein 1; Human Extracellular matrix protein 1 ELISA Kit; ECM1/p85/Secretory Component P85/extracellular matrix protein 1/Secretory Component Glycoprotein; ECM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ECM1. No significant cross-reactivity or interference between ECM1 and analogues was observed.
• Sequence Length: 415

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ECM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ECM1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECM1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ECM1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ECM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ECM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ECM1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2654435
• Molecular Weight: 63,563 Da
• NCBI Official Full Name: extracellular matrix protein 1
• UniProt Protein Name: Extracellular matrix protein 1
• Protein Family: Extracellular matrix protein
• UniProt Gene Name: ECM1
• UniProt Entry Name: ECM1_HUMAN

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: enzyme binding; interleukin-2 receptor binding; laminin binding; protease binding; protein binding; protein C-terminus binding; signal transducer activity

Biological Process: angiogenesis; biomineral formation; inflammatory response; negative regulation of bone mineralization; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of peptidase activity; ossification; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of T-helper 2 type immune response; regulation of transcription from RNA polymerase II promoter; signal transduction

Disease: Lipoid Proteinosis Of Urbach And Wiethe