Chicken Extracellular Matrix Protein 1 ELISA Kit | ECM1 elisa kit

Chicken Extracellular Matrix Protein 1 ELISA Kit

Cat. Num. AAA740791

• Reactivity: Chicken
• Synonyms: Extracellular Matrix Protein 1; Chicken Extracellular Matrix Protein 1 ELISA Kit; ECM1 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ECM1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This ECM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken ECM1. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY ECM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ECM1 antibody and an ECM1-HRP conjugate. The assay sample and buffer are incubated together with ECM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECM1 concentration since ECM1 from samples and ECM1-HRP conjugate compete for the anti-ECM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ECM1 from the sample, fewer sites are left to bind ECM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECM1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ECM1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 2654433
• NCBI Official Full Name: extracellular matrix protein 1
• Protein Family: Extracellular matrix protein

Uniprot Description

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; positive regulation of endothelial cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; inflammatory response; regulation of T-helper 2 type immune response

Disease: Lipoid Proteinosis Of Urbach And Wiethe