Epstein-Barr Virus EBNA1 Recombinant Protein | EBV EBNA1 recombinant protein

Epstein-Barr Virus EBNA1 Recombinant

Cat. Num. AAA553169

• Applications: ELISA, Western Blot
• Purity: >90%
• Synonyms: Epstein-Barr Virus EBNA1; Epstein-Barr Virus EBNA1 Recombinant; ebna1; ebna-1; ebv; epstein barr; EBV EBNA1 recombinant protein

• Purity/Purification: >90%
• Form/Format: 0.2 um filtered 50mM Tris-HCI pH 8, 10mM glutathione, 60mM NaCI, 0.25% Sarkosyl and 50% Glycerol.
• Concentration: Bradford test: 7.14 mg/mL (varies by lot)
• Sequence Length: 641

• Applicable Applications for EBV EBNA1 recombinant protein: Suitable for use in ELISA and WB applications. Final concentrations to be determined by end user.
• Host Note: DNA sequence encoding fragment of EBV EBNA1 was expressed in Escherichia Coli.
• Preparation and Storage: This protein can be stored in working aliquots at 2-8°C for one month, or at -20°C for six months without detectable loss of activity.; Avoid repeated freeze/thaw cycles.

Related Product Information for EBV EBNA1 recombinant protein

EBNA1 is the only EBV protein consistently expressed in all proliferating infected cells and plays several important roles in EBV latent infection, including the initiation of EBV DNA replication, the mitotic segregation of the EBV genomes, and transcriptional activation of other EBV latency proteins. several pieces of evidence suggest that EBNA1 plays a direct role in cellular transformation by EBV. First, EBNA1 is expressed in all EBV-associated tumors and is the only viral protein expressed in some of these tumors. Second, transgenic mice expressing EBNA1 develop malignant B cell lymphomas. Third, the expression of EBNA1 in Hodgkin cells enhances their ability to form tumors in nonobese diabetic-SCID mice. Fourth, EBV genomes lacking the EBNA1 gene are several thousand-fold less efficient at B cell immortalization than EBV genomes expressing EBNA1. Fifth, interference with EBNA1 function in Burkitt's lymphoma cells by overexpression of the EBNA1 DNA binding domain increased cell death, suggesting that EBNA1 normally provides a survival function for these cells.

Product Categories/Family for EBV EBNA1 recombinant protein

Viral Antigens

NCBI and Uniprot Product Information

• NCBI GI #: 82503233
• NCBI GeneID: 3783774
• NCBI Accession #: YP_401677.1
• NCBI GenBank Nucleotide #: NC_007605.1
• UniProt Accession #: P03211; Q777E1; QQBE31
• Molecular Weight: Tested on SDS-PAGE: 45 kDa
• NCBI Official Full Name: EBNA-1
• UniProt Protein Name: Epstein-Barr nuclear antigen 1
• UniProt Gene Name: EBNA1
• UniProt Synonym Gene Names: EBNA-1
• UniProt Entry Name: EBNA1_EBVB9

Uniprot Description

Function: Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival. Ref.8

Subunit structure: Interacts with human USP7. Interacts with human EBP2; this interaction is important for the stable segregation of EBV episomes during cell division but not for the replication of the episomes. Interacts with BGLF4; this interaction facilitates the switch from latent to lytic DNA replication by down-regulating EBNA1 replication function. Ref.4 Ref.5 Ref.6 Ref.8

Subcellular location: Host nucleus Ref.3.

Sequence similarities: Belongs to the herpesviridae EBNA1 family.