Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Human Excitatory Amino Acid Transporter 1 (SLC1A3) ELISA Kit | EAAT1 elisa kit
Human Excitatory Amino Acid Transporter 1 (SLC1A3) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Background: Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. The cDNA encoded a deduced protein that was 95% homologous to a previously reported rabbit glutamate/aspartate transporter. The highest expression was found in the substantia nigra, red nucleus, and hippocampus, and in cerebral cortical layers
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
Uniprot Description
SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5p13
Cellular Component: cell surface; cell projection; membrane; cell soma; fibril; integral to membrane; plasma membrane
Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity
Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport
Disease: Episodic Ataxia, Type 6
Research Articles on EAAT1
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Product Notes
The Human EAAT1 slc1a3 (Catalog #AAA9714283) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714283 ELISA Kit recognizes Human EAAT1. It is sometimes possible for the material contained within the vial of "Excitatory Amino Acid Transporter 1 (SLC1A3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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